After the valve sonication procedure, it is suggested to purge the pump with water at 60 degrees, keeping the purge valve open. Weigh the sample into a syringe. Program the pump to purge the column with strong solvents. Purging has only helped to reduce. Dolan advises that this will clear. Plot the graph of Injection volume against area. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. Some pumps will require to manually open the purge valve and in others it is done via the software. isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC). Replace the O-Ring on the Purge Valve 150 Remove the Pump Head Assembly 153 Maintenance of a Pump Head Without Seal Wash Option 155HPLC troubleshooting guide Introduction During the development of a new HPLC method, there were unexpected results—all peaks, except for the. *Run to run RT variation of ±0. If problem still persists, contact system manufacturer. Currently loaded videos. GasesUltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. 05. Purge valve Purge function Remove the purge valve waste line and the capillary from the purge valve outlet. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. Over time, these frits will accu. If you see a major leak from the bottom of the pump head or in the gap between the two pump head, please contact your local Agilent support representative. This will redirect the solvent flow to the waste exit of the valve instead of passing through the whole system. Reducing the diffusion path to improve degassing. c. Replace piston seals. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. Retention times can shift from one run to the next – you need to know when it is ok and when it signifies a problem. First statement: Don’t let it run dry. From my little experience i learnt that it may be due to air trapped in. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. Vanquish Binary Pump F: Up to 1000 bar (15,000 psi) and 8 mL/min. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. 1. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. If using MassLynx or Empower. 1-15 of 17. KB003532: What is the procedure to purge / prime a quaternary pump? Purging the Pump The quaternary pump will be purged by opening the purge valve. On the front panel of the pumps, press the VP button. Make sure that you know which solvent is linked to which pump. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. You. Piston Housing of the Pump Head Assembly: The red arrows show the springs pushing back the pistons. Request Information. with a new bottle of HPLC-grade water. Yes it looks like this! Now the valve is ok and there’s no more leak, maybe now the frit is soaked by the mobile phase and fits better?! Thanks!! reply from dmarchini< dmarchini >…. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications,Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. Pump 15 minutes at this pressure to wear in the seals. If problem. (Prime each pump head separately. And, remember, if you have a dual-piston pump, you have two piston seals to replace. ID stainless steel tubing kit • Galaxie Chromatography Software-0. ) If system has check valve, loosen valve to allow air to escape. To start purging, press the "Purge" bottom on both LC-20AD and SIL-20AC. 7. • Clean the bottle head assembly using lint-free wipes to minimize solvent carryover. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. 0:44. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. Replace PTFE frit in the purge valve. 18: Ready Check Results window appears after the flow meter purge function is If the conclusions from the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX, and performing the Pump Leak Rate Test, points to a defective or clogged AIV, you may try the following procedure: How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pumps. Between samples when your compound is very sticky (apolar) to help clean the column. 9. Search by lot number or partial lot number. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). 5 ml/min for 4 min, I only collected 1 ml of liquid. Request a Certificate. Fig. Flexible Pump The Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump. This video shows the operation a HPLC purge valve. 1:55. , 5-10 mL/min. 3. , 10 mL/min) to clear all the air from the pump and connecting tubing. HPLC pumps can be single, binary, or ternary, meaning that the can make use of one, two, or more different solvent reservoirs. categories. Santa Clara, CA 95051. Press “Menu” till the purge appears on the screen. Purge pump at high flow rate (e. Pump a 20% nitric acid/water solution through the pump for 3 minutes. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Close the purge valve, set the flow to a value that gives a pressure of 350 bar. L/mni for 5mni . With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. Turn the black valve anticlockwise to purge the mobile phase. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. The stationary phase is a granular material with very small porous particles in a separation column. DO NOT PURGE THE SYSTEM WITH THE BALL VALVE CLOSED! THIS WILL RESULT IN PRESSURE TOO HIGH FOR THE PUMP TO HANDLE AND CAN RUIN THE COLUMN!!! 6. Products. Focus on the column's inlet: After the initial flush, reduce the flow rate to 1 mL/min or so and close the purge valve until no more bubbles emerge. If adequate purging cannot be achieved with auto-purging or a suction tube does not contain liquid, for example, use the following procedure to manually purge with a syringe. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’. 5 um columns, use 0. Press the Inject button in Empower to wait for injection. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. Confirm that the lever is in the INJECT position, insert the syringe all the way into the injection port, and quickly switch the valve to the LOAD position. g. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. 0*Steps to follow: To backflush the needle seat according to the following procedure, you need a pump with a manual purge valve. This sets the mobile phase composition to 100% water so we can purge the water line. . 000 ml, – 25 psi. g. During step 1, check that the solvent moves into the solvent line correctly and it goes out the Purge Valve. ) If system has check valve, loosen valve to allow air to escape. Industry-leading flow and gradient precision. Draw syringe back to prime. 0 hours and also use 50% Methanol after water flushing. Seat backfl ush is enabled by a second pump, an integrated high-pressure fl ush pump. Press “Prime” button (or pump at max flow), continue to draw on syringe until no bubbles are seen. (Most ternary pumps actually have four solvent reservoirs. First, dissolved gasses in the mobile phase must diffuse to the surface of the membrane or film. 0. Draw approximately 20 mL of fluid. This video demonstrates the proper procedure to manually prime the pump on a Shimadzu LC 40 instrument. Residues or contaminations may block filters or capillaries. 6. High Performance Liquid Chromatography (HPLC) is an analytical technique that is ubiquitous in labs across the globe. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. Some bubbles may be generated from the suction filter head dipping in the mobile phase. 6. For reverse-phase columns, wet the column using a mobile phase like methanol and bring it to normal flow rate. † Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision, and accuracy. *Run to run RT variation of ±0. 88 Service Handbook for 1050 Series of HPLC Modules - 11/2001 Pumps: General Information Specifications Specifications Table 2 Specifications of 1050 PumpsHi,when I do wet prime/dry prime in hplc ? Thank you. 5. Performing the Ramp and Decay Test After a PM. Kata kunci / keywords: HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product. A damping unit is connected between the two plunger chambers. If the pressure does not rise, the purge valve needs to be replaced as it was the cause of high pressure. Use only HPLC-grade solvents and water filtered through 0. Figure 6: Pump head with purge valve and outlet to autosampler. 8. Figure 2 shows how to perform the purge for an i-Series. 1. 1:55. Perform flow rate check. Flush the column with 10/90 water/ACN for 5 min at 1. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. e. g. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. 23 MPa. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. SPE, LLE) on. 6 mm ID columns over the flow rate range up to 5 mL/min. Always do this one channel at a time (e. 02 in. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. This pump enables quaternary solvent delivery at pressures up to 800. Remove the LC tubing from the HPLC column and insert the tubing into a waste container. 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA™ avant system. Left pump connected to Vector PC post column derivatization. The Vanquish HPLC and UHPLC systems offer uncompromised performance, robustness, and ease of use. 4. Turn the black valve anticlockwise to purge the mobile phase. Overall Routine Pump Maintenance 1. ResearchGate | Find and share researchpump for extended seal lifetime † Setting upper and lower pressure limits will turn off the pump, e. The display will show, 515 Pump, 100 µl, Ver 1. If the pressure reaches its maximum (press. Meet Your New Lab Ally - the Alliance iS HPLC System. 1 ml/min and connect the size exclusion column (4. 45 or 0. Page 103 • Operation Figure 29: Dwell volume. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is zero; Analysis. Purge HPLC pumps with buffer made in step 1. September 2, 2013 . Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. c Enter the new parameters in step 8, and click OK. 3. 3. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. 6. Turn . Loss of Power. The system must be primed to remove air bubbles and for the pump to function properly. you will need to • General cleaning Daily first re-prime the pump by purging the air from all the lines. HPLC Pumps. 7:30. Read Less. High Pressure Liquid Chromatography. Steps to follow: Store the column in an appropriate solvent (check column information sheet) When you remove the column, add a column union so the detector gets flushed as well in the following steps. For. If seal wash option. 05 0 0. Always do this one channel at a time (e. Pump problem – Contact your maintenance provider. Cause a:Air bubble in pump head. Basic HPLC System Components. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. 6 Withdraw ~10ml of water and close the purge valve. Excellent gradient performance resulting from the highly accurate solvent delivery by the 5110/5160 pump with Hitachi’s unique high-speed. The mobile phase, on the other hand, is a solvent or solvent mixture which is forced at high pressure through the separation column. Count on the Arc HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence. then you have a little bit of work to do to really prime the line as that pump is not really powerful enough to draw in liquid if it is super dry. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. 5:26. High Performance Liquid Chromatography (HPLC) is an analytical technique that is ubiquitous in labs across the globe. Gradually increase system flow up to the required flow rate,. 02-0. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. 1. A pressure sensor monitors the pump pressure. How do i manually prime/purge it? Because there are some air bubbles, and if they get inside the needle, its gonna be bad news bears ( i think). 05 MPa or 0. L/mni for 5mni . 2. b. Up to 6 FRC-40’s can be configured. 8 Click pump on the flow diagram and change the flow path into A2 and B2. 1:59. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. If the preceding step does not solve the issue, remove the purge valve and start the pump. Open the "drain" on LC-20AD. g. Almost all descriptions in the manual apply to all pump models in the LPG-3000 pump series and cover both the standard (stainless steel) and biocompatible pumps. 결과 및 정리. 3. 6. The system pressure and PreColumn pressure also fluctuates between 0. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. If using MassLynx or Empower software, refer to the PDFs entitled Priming the 1525 Binary HPLC Pump Using MassLynx or Priming the 1525 Binary HPLC Pump Using Empower. 322 HPLC PumP | uSER’S GuIDE 13 De SC r I pt ION pUMp heaDS Two interchangeable pump head types are available:Passivation is a chemical treatment of stainless steel and purging is a cleaning process. 0:44. Set the flow rate to 5 ml/min d. Maintenance Kits for UltiMate™ 3000 Pumps. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. )• Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. If problem persists, flush system with 100% methanol or isopropanol. 2 Set the flow of the pump at 1ml/min. Set flow to 0ml/min f. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. e. This is a prevention step. 결과 및 정리. 4. Air or particulates in head of pump or valves of the pump Leakage in transmission lines Solution • Avoid formation of negative pressure, utilisation of equalising valves in the caps of the eluent flask, prepare fresh buffer • Check tightness, if necessary, replace seals or valves • Purge the pumps with water or IPA, flush waterWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Purification Solvent Management. Pump 100% isopropanol through the pump for 3 minutes. Vent. When the purge valve is open, solvent from the quaternary pump will go straight to waste rather than proceeding through the column. (Prime each pump head separately. TP01408 RUN STOP EDIT ENTER MENU Waters 515 HPLC Pump Draw-off valve Seal wash hole (not visible) Pump head assembly High. Moreover, if the faulty purge valve completely blocks the engine, it cannot start or run. An autosampler works by aspirating and injecting a precise sample volume into the mobile phase stream. The impact of this type of clogging will be gradual, not sudden. Purge pump at high flow rate (e. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. 2 1. Open prime/purge valve (in the case of Chrom Tech pumps, you turn the knob counterclockwise one to two turns. If a purge valve is not functioning correctly, it can lead to the vehicle losing power or stalling unexpectedly. 6 Purging the Pump. Set a flow of 5 ml/min and purge each channel for 5 min with HPLC-quality Water. The system pressure and PreColumn pressure also fluctuates between 0. Purge valve open. 1 A) Calibration of Pump:-. Waters Second Quarter 2023 Highlights. As well as excellent accuracy, it has very small delay volumes of ≤ 350 μL. If I run the system at 0. Purge pump and attempt to clean check valves. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. Before using an HPLC system, you should prime all of the lines in your HPLC pump. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Remove and clean pistons. If seal wash option is installed, replace wash seals and gaskets. Click on Method 1. Open the purge valve by turning the knob on the front of the pump to the left until you feel some resistance. The dual piston design has minimal pulsation for stable baselines. Check that appropriate mixingThe 322 HPLC Pump is a multi-solvent pump for analytical to semi-preparative high performance liquid chromatography (HPLC). • Vacuum chambers – Contain the gas-permeable eluent channel. 4411-EN-2. 8. P680 HPLC PumpsPurge pump at high flow rate (e. Reciprocating pump, syringe pump, and pneumatic pump. See your pump modules user manual for detailed instructions on re-assembly. The EVAP system is designed to prevent fuel vapors from escaping into the atmosphere by capturing and temporarily storing them in a. The 1290 Infinity II Flexible Pump combines highest performance, methods compatibility, and. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. 4. 3:36. g. More lab efficiency with your auto delivery schedule, modify and cancel it. the user. Air bubbles can create such flow anomalies. Varian Modular Analytical HPLC Systems Figure 1. The binary Semipreparative High-Pressure Gradient P680 Pump is ideal for small-scale purification applications. † Minimizes carry over for viscous samples, minimum injection volumes, maximum sensitivity and if the Wellplate Autosampler is. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. Use volatile pH modifiers as one would use for LCMS mobile phases, like formic acid, acetic acid, ammonium formate, ammonium acetate, or ammonium hydroxide. ) If system has check valve, loosen valve to allow air to escape. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies courses. Perform search. 7. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. HPLC system. Check for signs of leaks. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. Eluent may then be allowed to progress through the IC system, and the pressure monitored. 2. Run each pump for 2 minutes. Here’s a trick: you can set a minimum pressure on your HPLC pump. ANSWER. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. It is column chromatography. Documents & Downloads. The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons. Basic HPLC System Components. Agilent University. 8. Properly shut down all the HPLC modules from the front (LC-20 and LC-30) or backUltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMThe PTFE frits in your Agilent LC pumps will need to be replaced from time to time as part of a routine maintenance program. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. flow rate) One of the most important performance measures for HPLC is retention time reproducibility. 2. Therefore, to estimate how much volume is required for a purge, check the volume specification of the inlet tubing, the degasser and the pump that you are using, then double it to give an approximate purge volume. Make sure that the AVR7-3 inject valve is in the Purge position. Mobile phase is passed through porous polymer tubing placed in a vacuum chamber. Before the actual compression process begins (see Fig. 1:55. Priming the 1525 Binary HPLC Pump Using Empower When to Prime Priming and purging is necessary to ensure proper pump operation when: • You start the pump for the first time • You change eluent • The system has been idle for some time Parts Required None Tools/Materials Required • Priming syringe (in Startup Kit; WAT010337) Procedure 1. max) right away, reduce the flowrate before checking. Each page is marked with a tab and a footer to help you access information. Figure 6: Pump head with purge valve and outlet to autosampler. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. HPLC Principle. The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. 1 Overview of HPLC. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. Turn pump to ‘On’ 7. Worldwide Numbers . 2:19. Delivering Scientific Insights to Improve Human Health and Well-Being. ), prime system if necessary. Use the keypad to type in the solvent composition for pumps A, B, C, and D. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Replacing a Check Valve Cartridge. • Syringe, loop & injector plunger seal. closed. 3 Page 5 Column Observations Potential Problems[][]Purge the pump with fresh diluent (filtered and degassed). Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting. 2:32. (You can press the same bottoms again to stop purging. 7. Achieve binary-like performance in your HPLC instrument. ), prime system if necessary. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. Close the purge valve. Pressure B. Pump heads with maximum flow rates of 10 and 50 ml/min are available. Open the black purge valve and press the “Purge” button. The 1290 Infinity II High Speed Pump has an exceptionally low delay volume, which allows you to run ultrafast. solvents. • Purge each channe lwtih 25–3m. Once the air is removed, close the valve and start flow. Operation of DAD: [][]Switch ‘ON’ the power of Quaternary Pump, Autosampler, Thermostatic Column Compartment, Detector, CPU, Monitor if already not switched on. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. An optional seal wash function is available for applications usingOpen the purge valve and set the flow rate of 5 mL/min for each channel with HPLC grade water. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. Press the EDIT/ ENTER key. between the degasser and the column. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. You. Open the manual purge valve or use the purge command, depending on the pump type. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. ID stainless steel tubing kit • Galaxie Chromatography Software-0. It also includes relevant guidance for practical handling and mainte-(1:27min) Exchanging the Purge Valve Frit or the Purge Valve (3. This small piece is meant to protect the rest of the system from particulates, and it needs to be replaced regularly. This video explains the reasons for pump priming. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. I am working with HPLC Shimadzu 20AD fitted with LC20AD. It is recommended to change your HPLC pump’s piston seals during planned preventative maintenance. 6.